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igg2b isotype control  (Bio X Cell)


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    Bio X Cell igg2b isotype control
    Igg2b Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg2b+isotype+control/InVivoMAb+mouse+IgG2b+isotype+control%2C+unknown+specificity/bio_rxiv__64898__2026__02__28__707759-37-42-47
    Average 94 stars, based on 250 article reviews
    igg2b isotype control - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Control:

    Article Title: Brd7 loss reawakens dormant metastasis initiating cells in lung by forging an immunosuppressive niche
    Article Snippet: For immune checkpoint therapy, the mice injected with the 4T07-TGL-Brd7 KO cells (Day 0), were treated with either the anti-CTLA4 ab alone (BioXcell, clone 9D9), anti-LAG3 alone (BioXcell, clone C9B7W) or the anti-CTLA4 plus anti-LAG3 combination intraperitoneally (300 μg/mice) on days 1,3,5 and on every alternate day thereafter until Week 5. .. The mice in the control group were administered (300 μg/mice) with mouse IgG2b isotype control (BioXcell, clone MPC-11) and the rat IgG1 isotype control (BioXcell, clone HRPN). .. For Sivelestat (MedChemExpress, HY17443) treatment, the mice injected with the 4T07-TGL-Brd7 KO cells (Day 0) were i.p. injected with Sivelestat (50 mg/kg) or DMSO control on days -5,-3,-1,1 and on every alternate day thereafter until Week 4.

    Article Title: A ligand-mimetic anti-TREM2 agonist antibody elevates soluble TREM2 and ameliorates pathology in mouse models of Alzheimer's disease and multiple sclerosis.
    Article Snippet: 265 ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Page 13 266 In vitro phagocytosis assay 267 6 x 104 cells/well of mouse microglial cell line BV-2 (AcceGen Biotechnology, 268 #ABC-TC212S) were plated in 96-well plates. .. The next day, cells were treated with or 269 without 30 nM anti-TREM2 antibody 03O05 or mouse IgG2b isotype control (msIgG2b, 270 bioxcell, #BP0086) for 2 to 24 hours, and 10 μM cytochalasin D (Invitrogen, #PHZ1063) 271 was included as a negative control. .. At the end of the treatment, the media was replaced 272 with Live Cell Imaging Solution (Invitrogen, #A59688DJ) containing 0.5 mg/ml pHrodo 273 Green E.coli BioParticles Conjugate for Phagocytosis (Invitrogen, #P35366), and cells 274 were incubated at 37°C without elevated CO2 for 3 hours.

    Article Title: Norovirus co-opts NINJ1 for selective protein secretion.
    Article Snippet: .. A 2- mg volume of total protein was mixed with 10 μg of anti- NS1 monoclonal antibody (2A9) or mouse IgG2b isotype control (BioXCell), which was preincubated with Dynabeads Protein G (Thermo Fisher Scientific) for 1 hour at 4°C. ..

    Article Title: Brd7 loss reawakens dormant metastasis initiating cells in lung by forging an immunosuppressive niche.
    Article Snippet: For immune checkpoint therapy, the mice injected with the 4T07-TGL-Brd7 KO cells (Day 0), were treated with either the anti-CTLA4 ab alone (BioXcell, clone 9D9), antiLAG3 alone (BioXcell, clone C9B7W) or the anti-CTLA4 plus anti-LAG3 combination intraperitoneally (300 μg/mice) on days 1,3,5 and on every alternate day thereafter until Week 5. .. The mice in the control group were administered (300 μg/mice) with mouse IgG2b isotype control (BioXcell, clone MPC-11) and the rat IgG1 isotype control (BioXcell, clone HRPN). .. For Sivelestat (MedChemExpress, HY17443) treatment, the mice injected with the 4T07-TGL-Brd7 KO cells (Day 0) were i.p. injected with Sivelestat (50mg/kg) or DMSO control on days -5,-3,-1,1 and on every alternate day thereafter until Week 4.

    Article Title: IL-6 underlies microenvironment immunosuppression and resistance to therapy in glioblastoma
    Article Snippet: .. Antibodies for in vivo experiments were: 250 μg of aPD-1 clone RMP1-14 (Bio X Cell, cat# BP0146), 250 μg of aIL-6 clone MP5-20F3 (Bio X Cell, cat# BE0046), and mouse IgG2b isotype control clone MPC-11 (Bio X Cell, cat# BE0086). .. Antibodies for in vivo experiments were: 250 μg of aPD-1 clone RMP1-14 (Bio X Cell, cat# BP0146), 250 μg of aIL-6 clone MP5-20F3 (Bio X Cell, cat# BE0046), and mouse IgG2b isotype control clone MPC-11 (Bio X Cell, cat# BE0086).

    Article Title: Adaptive immunity to retroelements promotes barrier integrity
    Article Snippet: .. For depletion of CD8 T cells, mice were injected intraperitoneally (i.p.) with 200 μg of anti-CD8 (2.43, BioXcell) or mouse IgG2b isotype control (LTF-2, BioXcell) daily for 3 days prior to the initial BHI-tween exposure. ..

    Article Title: Norovirus co-opts NINJ1 for selective protein secretion
    Article Snippet: .. A 2-mg volume of total protein was mixed with 10 μg of anti-NS1 monoclonal antibody (2A9) or mouse IgG2b isotype control (BioXCell), which was preincubated with Dynabeads Protein G (Thermo Fisher Scientific) for 1 hour at 4°C. ..

    Article Title: Liver cancer chronically exposed to palmitate acquires ferroptosis resistance via the downregulation of glutamine-driven hepcidin expression.
    Article Snippet: Background: Immune checkpoint blockade (ICB) has revolutionized treatment of hepatocellular carcinoma (HCC), but its efficacy remains limited.. Recent studies demonstrate that resistance to ferroptosis is a significant barrier to the success of ICB.. Methods: Ferroptosis was assessed by measuring C11-BODIPY fluorescence and 4-hydroxynonenal (4-HNE) staining.

    Negative Control:

    Article Title: A ligand-mimetic anti-TREM2 agonist antibody elevates soluble TREM2 and ameliorates pathology in mouse models of Alzheimer's disease and multiple sclerosis.
    Article Snippet: 265 ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Page 13 266 In vitro phagocytosis assay 267 6 x 104 cells/well of mouse microglial cell line BV-2 (AcceGen Biotechnology, 268 #ABC-TC212S) were plated in 96-well plates. .. The next day, cells were treated with or 269 without 30 nM anti-TREM2 antibody 03O05 or mouse IgG2b isotype control (msIgG2b, 270 bioxcell, #BP0086) for 2 to 24 hours, and 10 μM cytochalasin D (Invitrogen, #PHZ1063) 271 was included as a negative control. .. At the end of the treatment, the media was replaced 272 with Live Cell Imaging Solution (Invitrogen, #A59688DJ) containing 0.5 mg/ml pHrodo 273 Green E.coli BioParticles Conjugate for Phagocytosis (Invitrogen, #P35366), and cells 274 were incubated at 37°C without elevated CO2 for 3 hours.

    In Vivo:

    Article Title: IL-6 underlies microenvironment immunosuppression and resistance to therapy in glioblastoma
    Article Snippet: .. Antibodies for in vivo experiments were: 250 μg of aPD-1 clone RMP1-14 (Bio X Cell, cat# BP0146), 250 μg of aIL-6 clone MP5-20F3 (Bio X Cell, cat# BE0046), and mouse IgG2b isotype control clone MPC-11 (Bio X Cell, cat# BE0086). .. Antibodies for in vivo experiments were: 250 μg of aPD-1 clone RMP1-14 (Bio X Cell, cat# BP0146), 250 μg of aIL-6 clone MP5-20F3 (Bio X Cell, cat# BE0046), and mouse IgG2b isotype control clone MPC-11 (Bio X Cell, cat# BE0086).

    Injection:

    Article Title: Adaptive immunity to retroelements promotes barrier integrity
    Article Snippet: .. For depletion of CD8 T cells, mice were injected intraperitoneally (i.p.) with 200 μg of anti-CD8 (2.43, BioXcell) or mouse IgG2b isotype control (LTF-2, BioXcell) daily for 3 days prior to the initial BHI-tween exposure. ..



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    Image Search Results


    A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

    Journal: bioRxiv

    Article Title: Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks

    doi: 10.64898/2026.03.26.711344

    Figure Lengend Snippet: A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

    Article Snippet: Cells were stained with CD24 (Miltenyi Biotec, 130-108-352) or isotype (Miltenyi Biotec, 130-124-062) antibodies at the manufacturer’s suggested concentration for 20 minutes at 4°C, washed and acquired using a MACSquant YVB.

    Techniques: Binding Assay, Flow Cytometry, Activity Assay, Staining, Control